Jason Scovell

5 Questions 5 Answers 0 Followers

Questions related from Jason Scovell

When doing Golden Gate Cloning why do I get many negative colonies?

I was wondering if someone could point me in the right direction on what I am doing wrong. I am trying to clone in 3 vectors to the destination vector pFus_A (I am not doing this for TALEN). I...

12 February 2015 9,371 5 View

Where is the 'center' of my HDR construct for CRISPR (point mutation) - Cas9 cut site vs. point mutation?

Seems like there are lots of resources about the nuances of generating gRNAs, but a little less on designing HDR constructs. Just a few quick questions: 1. If my gRNA is ~50 bp away from where my...

15 November 2014 2,923 9 View

Are these spheroid or just cell clumps?

Hey guys, I am trying to generate 'nephrospheres' from mouse kidneys, but I am uncertain if I am just getting cell clumps or if you think these are fairly representative of spheroids? As a...

14 November 2014 3,311 6 View

Can anyone help with DIG Southern Blot?

We have been troubleshooting the DIG-labeled southern blotting kit from Roche with no success. Here is a little background on what we have been able to achieve and what we have tried. We are...

28 April 2014 1,039 1 View

Plasmid extraction in ABLE-K cells (low copy)?

I am having difficulty with purifying plasmids from ABLE-K cells. I have had no problems using qiagen mini prep kits in the past for other e. coli strains. It is my understanding that ABLE-K cells...

12 February 2014 4,127 0 View