Jason Chou

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Questions related from Jason Chou

What is the best way to obtain high quality purifications of mouse monoclonal IgG1/λ?

Hi Everyone, we are trying to purify a mouse monoclonal IgG1/λ for a p24 sELISA system. From several experiments we have determined this antibody to be the bottleneck in increasing our...

11 November 2014 9,727 3 View

Causes of high background in sandwich ELISA with no antigen?

I am trying to optimize a sandwich ELISA with a p24 protein standard, and I'm getting very high background (0.4-0.5) in wells that have no antigen. These wells consist of only Capture mAb ->...

22 October 2014 7,931 15 View