Does anyone have some experience with CRISPR Cas9 to knock in a gene with a point mutation which leads to a single amino acid substitution?

Within my project I want to use CRSIPR Cas9 to generate stable knock-in cell lines. The goal is to knock in a gene with a point mutation which leads to a single amino acid substitution. I read a...

24 September 2014 1,462 5 View