Jasmin D'Andrea

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Questions related from Jasmin D'Andrea

While blotting for S-nitrosylation using the Pierce western blot kit, is it necessary to use the recommended desalting column?

About to attempt to blot for Nitrosylation using the Pierce S-Nitrosylation Western blot kit. In the protocol, they suggest to precipiate the protein in 6x volume of acetone for 1 hour. They also...

06 June 2019 3,944 1 View

Now do you dissolve S-Nitroso-N-Acetyl-D,L-Penicillamine (SNAP) for cell culture use?

Looking to use SNAP for some melanocyte cell work. The protocol sheet says to use deoxygenated buffers to make stocks but it also says it is soluble in PBS. So why do you need to go through the...

06 June 2019 6,618 2 View