Jasmin Baby

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Questions related from Jasmin Baby

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

18 February 2021 9,346 2 View

What are the points to keep in mind to create a bi-allelic deletion using Crispr/cas9 plasmid : PX459, PX458 ?

I was just going to start an experiment with the PX458 and PX459 plasmids for the deletion of an 800bp long gene segment. These plasmids, somehow, seem to create clones with heterologous deletions...

16 January 2019 1,238 1 View