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Questions related from Jaroslav Michalko
I tried ApE software, but there you can define just one ORF, I need to have intron sequence incorporated in the sequence. I want to avoid making it manually.
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What is the purpose of adding the DTT into the SDS-loading buffer just before use? Is it wrong to mix together all substances (i.e. with DTT) and then store them at -20 °C for a prolonged time?
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