Can I get a gene expression result in qPCR if the forward primer binds to the exon junction but the reverse primer binds to the UTR?

That gene has four transcripts. To experiment with gene expression, I have designed a qPCR primer from NCBI. But it gives me primer pairs, of which forward primer binds to the exon-exon junction...

17 April 2024 6,477 2 View