Jana de Sostoa

2 Questions 2 Answers 0 Followers

Questions related from Jana de Sostoa

In vitro human M1 and M2-macrophage differentiation problems, why?

Dear all, I am currently doing human macrophage differentiation and polarization (to M1 and M2) from CD14+ monocytes. I isolate CD14+ monocytes from human PBMCs with miltenyi beads, then I...

28 May 2021 5,655 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

03 November 2020 8,862 3 View