Jan Čapek

3 Questions 2 Answers 0 Followers

Questions related from Jan Čapek

QPCR data analysis - student's t test with relative expressions?

Hi qPCR geeks :-)  Since being unexperienced researcher (generally) and especially in the field of the analysis of my qPCR data I have run into problem for which I could not find a definitive...

09 October 2019 9,366 1 View

Is my RNA degraded?

Hi. In an attachment I am sending picture of a gel after my RNA isolation. Since I do not have any experience with RNA isolation I am somewhat confused in interpreting what could possibly happen...

16 December 2016 1,995 4 View

Will be protein forming inclusion bodies visible on SDS-PAGE gel?

I prepared cell lysate by sonication and also by heating (10 min, 95 C) with Laemli sample buffer (SDS and DTT present) and then I did SDS-PAGE. My question is: Will I see on that gel also protein...

01 May 2016 6,946 6 View