Jamie Newton

2 Questions 3 Answers 0 Followers

Questions related from Jamie Newton

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

15 February 2021 3,058 2 View

Blastocystis PCR - Agarose gel

Doing a nested PCR for Blastocystis useing primers RD3 and RD5 for the first PCR and primers RD5F and BrRDr for the second PCR. I'm getting bands on the gel but also getting a lot of smearing....

01 January 1970 3,358 3 View