James Reeve

2 Questions 2 Answers 0 Followers

Questions related from James Reeve

Will a low A260/A230 affect Illumina next-gen sequencing?

I'm currently preparing DNA from six populations of fish for whole genome sequencing on an Illumina HiSeq machine. Unfortunately half my populations have a low A260/A230 score (~1.6) indicating...

06 June 2018 5,187 4 View

Should I have a PCR step for pool-seq?

I'm preparing samples for pool-seq and am currently weighing up library prep options. I've heard that PCR introduced bias in pool-seq, as some individuals may be replicated more than others. This...

04 April 2018 408 5 View