James Prescott

2 Questions 2 Answers 0 Followers

Questions related from James Prescott

What is a good set of 16s primers to quantify total number of bacteria in a sample?

I'm looking for a pair of 16s primers to use in a SYBR green qpcr assay to quantify the total amount of bacteria in a sample to compare to my species specific assays. Do you have any suggestions?

01 January 2019 8,178 6 View

I'm getting different melting points for my positive controls and unknowns in my V. vulnificus SYBR green qpcr assay, how can I troubleshoot this?

I'm using SYBR iQ supermix and the vvha primers described here: Real-Time PCR Analysis of Vibrio vulnificus from Oysters I'm not sure if there is an issue because these primers are for a probe...

08 August 2018 8,597 2 View