Methyl Green staining cytoplasm and doesn't appear specific. Tips to improve counterstain specificity?

Using Methyl Green as a counterstain on DAB chromogen stains; IBA1 and GFAP. The Methyl Green solution is 0.125% VWR brand powder, made in 0.1M Sodium Acetate buffer at pH ~.4.2-4.3. The tissue is...

28 November 2020 3,816 2 View