James Saga

5 Questions 4 Answers 0 Followers

Questions related from James Saga

Can anyone share their first strand cDNA synthesis protocol?

I have a kit from Roche (link: https://lifescience.roche.com/en_us/products/transcriptor-first-strand-cdna-synthesis-kit.html) but i cannot find a protocol anywhere. I have a limited amount of RNA...

15 September 2021 4,536 3 View

Cloning is not working. Every step seems OK but the final product is very odd. Help?

Hello. I am trying to clone Jag1 into pGBKT7 vector, but for some reason it does not work and we are very puzzled by our results. Below is everything I did from start to finish: Jag 1 containing...

12 May 2021 6,220 1 View

Does the color of bacterial pellet indicates something during cloning?

Hello Everyone. I've been having trouble with cloning. I do get colonies but when I precipitate DNA I do not get a band for any plasmid on the gel. in Fact I am getting faint genomic DNA bands...

05 May 2021 3,997 5 View

Can I substitute MnCl2 with MgCl2 when making competent cells?

Hello. I am trying to make competent cells and the transformation buffer in my protocol requires MnCl2. I don't have it, but I do have MgCl2 and am wondering if it will work the same. The...

17 February 2021 9,229 3 View

How to stain adherent cells with DAPI?

Hello! I am growing transfected cells in 24 well plate. on the bottom of each well i have a small glass cover-slip. so the cells adhere to that cover slip. I am using this method because its very...

16 December 2020 7,328 3 View