James Ropa

3 Questions 16 Answers 0 Followers

Questions related from James Ropa

CA and GT repeats consistently being shown as overrepresented sequences in Novaseq datasets. Is this an artifact of the sequencing technology?

Recently, I have sequenced many libraries on Novaseq. I am aware of the dark cycles causing long polyG reads due to Novaseq's two color technology. However, I am also seeing pretty consistent...

24 January 2020 3,994 2 View

Is there a way to stabilize RNA before FACS followed by RNA harvest for RNA-sequencing for very low cell numbers?

I am currently trying to isolate a small number of cells (200-500 cells per sample) using FACS. I sort the cells directly into an eppendorf tube containing RLT plus lysis buffer (Qiagen) with 1%...

11 November 2019 1,799 5 View

Is there a method of determining the overall accessibility of DNA, similar to ATAC-seq, but without having to actually do sequencing?

In looking at massive changes in the epigenome, we will sometimes do histone extractions followed by western blot looking at specific histone modifications to determine whether they change on a...

09 March 2018 6,482 5 View