James Jeffrey Morris

2 Questions 4 Answers 0 Followers

Questions related from James Jeffrey Morris

Where can one get a simple BAC/fosmid backbone for large-fragment cloning?

I'm trying to clone a large operon (30kb) for expression in E. coli. I'm concerned that if I put this into a high-copy number plasmid it won't be stable. My first thought was to use a BAC or a...

18 October 2013 7,745 0 View

Is there a cultured bacteriophage capable of infecting Synechococcus PCC 7942?

Looking for a phage that can infect the "green E. coli." Would like to do some studies of co-existence in a system amenable to genetic modification. Have tried isolating from nearby lakes...

30 November 2012 4,222 5 View