James Seun Maku

2 Questions 3 Answers 0 Followers

Questions related from James Seun Maku

Do anyone have a protocol of using a hydroponic nutrient solution for transformant selection using hygromycin? Not on agar but in hydroponic solution?

Please I need to know how to add hygromycin B to my hydroponic solution for transformant selection. My vector construct contain Hygromycin ressistant gene (HPT). I know how to use the MS selection...

16 November 2016 2,089 4 View

I am using Agrobacterium rhizogene to transform some Arachis and gene knockout using pYLCRISIPR/Cas9?

I'm working on Peanut nodulation gene knockout using pYLCRISIPR/Cas9... One set using culture media (co-culture) and (selection media supplemented with hygromycin B) since the Binary plasmid has...

14 March 2016 1,502 1 View