James Lrod

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Questions related from James Lrod

How to cause hydrolysis of GTP bound to tubulin dimers without causing polymerization?

I am trying to get pure GDP-bound tubulin. Presumably, ordering purified tubulin from bovine or porcine brain will have a mixture of GDP and GTP bound tubulin. I really need a pure GDP-bound...

30 August 2023 8,317 0 View

Possible causes of non-specific binding of primary antibody but none to my protein of interest?

So I have been having a lot of issues detecting a particular protein via western blot. When I image the blot I get a lot of clear bands going all the way up the lane, almost looking like a...

24 May 2023 8,493 3 View