James Hillier

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Questions related from James Hillier

Protocol for stable lentiviral transduction of U937, HL60 or K562 cell lines?

Does any one have any experience transducing U937, HL60 or K562 cell lines with lentivirus. I am using the plenti-c-myc-ddk-ires-puro vector from Origene...

18 October 2017 9,577 3 View

What template can I use to check the amplification efficiency of my endogenous control/house keeping gene in qPCR?

I am performing qPCR and want to check the amplification efficiency of my target and housekeeping genes. I have a plasmid with my target gene in that I think can be used as a template for this...

28 June 2017 4,348 2 View