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Questions related from James Bruns
Are there any distinguishing features of macrophages that I can use to identify them in skin tissue on TEM images without any kind of labeling?
13 September 2022 7,977 0 View
I am learning how to do negative staining on cells for TEM, and I am seeing highly variable shapes/arrangement of nuclei. Is this normal, or did something go wrong with the processing?
10 June 2022 7,670 3 View
The lab I work in is looking to isolate phages from water sources, but we have had no luck in the past. What is the best protocol for isolating phages from water samples? We work primarily with...
30 January 2022 269 1 View
What is better to observe organelles and chromatin? Embedding cells in resin or cryo preparations for TEM?
04 December 2021 136 3 View
I am trying to make fixative for transmission electron microscopy of our fibroblast, and for some reason my solution goes cloudy when it should be clear. I was given a protocol to work off of by...
04 December 2021 1,548 0 View
I was wondering what number of cells is best for a pellet for TEM sample processing. I believe the best pellets are ~ 1mm^3 in literature, but I wanted to know what worked best for everyone else.
07 November 2021 2,135 4 View
