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Questions related from James Bruns
I am learning how to do negative staining on cells for TEM, and I am seeing highly variable shapes/arrangement of nuclei. Is this normal, or did something go wrong with the processing?
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What is better to observe organelles and chromatin? Embedding cells in resin or cryo preparations for TEM?
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I am trying to make fixative for transmission electron microscopy of our fibroblast, and for some reason my solution goes cloudy when it should be clear. I was given a protocol to work off of by...
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