James Barnett

3 Questions 8 Answers 0 Followers

Questions related from James Barnett

How do I increase the stromal (cancer-associated fibroblast) content in murine tumor models?

I have screened several tumor models (Panc02, 4T1, E0771 and EMT6) for CAF markers (such as FSP-1 and PDGF receptor) through flow cytometric analysis - the population of live CAFs in each model...

08 April 2021 1,715 10 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

09 February 2021 976 6 View

What is the viability of MDSCs after freezing?

My plan is to isolate immature myeloid cells (IMCs) from the spleen and to expand them into MDSCs with IL-6 and GM-CSF supplemented media. I have successfully isolated splenocytes from...

04 October 2019 285 3 View