James Barnett

4 Questions 10 Answers 0 Followers

Questions related from James Barnett

How do I increase the stromal (cancer-associated fibroblast) content in murine tumor models?

I have screened several tumor models (Panc02, 4T1, E0771 and EMT6) for CAF markers (such as FSP-1 and PDGF receptor) through flow cytometric analysis - the population of live CAFs in each model...

08 April 2021 1,768 10 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

09 February 2021 1,177 6 View

What is the viability of MDSCs after freezing?

My plan is to isolate immature myeloid cells (IMCs) from the spleen and to expand them into MDSCs with IL-6 and GM-CSF supplemented media. I have successfully isolated splenocytes from...

04 October 2019 351 3 View

Abundance of tumor-derived myeloid-derived suppressor cells (MDSC) in mouse models

There are a few kits that can be used to isolate MDSCs from mouse tumors such as the EasySep system (Stem Cell). I want to know if MDSCs are present in high abundance in tumors in order to plan...

01 January 1970 6,667 4 View