Should I use an ssODN template that is sense or antisense to my sgRNA when performing HDR mediated gene repair with CRISPR?

I would like to perform a specific deletion (single nucleotide deletion) using CRISPR/Cas system. I add a repair template in form of ssODN oligo (150bp long) to mediate a HDR repair. One thing is...

26 March 2015 905 19 View