Jakob Leppkes

3 Questions 5 Answers 0 Followers

Questions related from Jakob Leppkes

What can I do to get a straight baseline in RP-HPLC?

I am running a RP-HPLC system with a C18 column. In our group, we mainly use the system to analyze peptides. The baseline of the system is not stable. In every run it is increasing over time. The...

30 July 2020 5,188 11 View

Which tube should I use to connect vacuum oil pump with Schlenk line?

I want to connect the vacuum oil pumps in our lab with the Schlenk lines and I am not sure which tubes (material, brand, etc.) I should choose. At the moment I am using a red rubber tube and it is...

31 January 2020 7,491 2 View

How is folding of cysteine-rich protein with GSH/GSSG carried out successfully?

I'm trying to refold the cysteine-rich bovine pancreatic trypsin inhibitor (BPTI) with a glutathione(reduced) / glutathione(oxidized) - redox system. I use a Tris-buffer at pH 8. First I reduced...

11 October 2018 9,256 2 View