Jaison Jeevan Sequeira

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Questions related from Jaison Jeevan Sequeira

Are faulty cryopreservation procedures responsible for cells to lose gene expression?

We had recently re-organized the cell lines in our cryo can (Liquid nitrogen container) by shifting all the canisters into a new can. This process took about 10-15 minutes. Now, all the cell lines...

05 May 2017 1,894 6 View

A set of stably expressing HEK293 cells showed a consistent low hERG tail current during patch clamping. Possible reasons?

Does it mean that the cells have lost hERG gene expression? Does cell confluency and cell density while freezing affect the cell line characteristics? What is the role of selection antibiotic...

04 April 2017 5,917 3 View