What is the reason for not getting colony during transformation of PfapHeli gene inserted into pet28a vector into Rosetta2DE3?

I used codon optimized Plasmodium falciparum apicoplast helicase gene inserted into pet28a vector system (from Genscript) transformed into Rosetta 2DE3. I did transformation for 3-4 times 1-2...

01 July 2021 7,932 3 View