Jai krishna Mahto

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Questions related from Jai krishna Mahto

For 10 ns md run in gromacs; should I increase dt or steps?

Run parametersintegrator = md ;nsteps = 5000000 ; 2 * 5000000 = 10000 ps (10 ns)dt = 0.002 ; 2 fs This is the way I am running md simulation currently. is it correct?

06 June 2017 9,513 2 View

Protein is not getting purified. why?

We have a protein of 40kDa size with his tag. purification with Ni-NTA column showed impurities. when gel filtration was run after ni nta column chromatography, again same bands appeared on SDS...

04 April 2017 8,937 5 View

Restriction digestion product is smear. what is the cause behind it?

I digested bacterial plasmid with EcoRI and EcoRV. digested product was smear. But the plasmid has only two RE site.

12 December 2016 6,705 5 View

Does particular restriction sites are preferred over other?

I am digesting plasmid which has two EcoRI RE site. But I am not getting two bands after digestion.

11 November 2016 5,824 0 View

Can we run hexane extracted sample in HPLC with acetonitrile as mobile phase?

I want to run HPLC for the hexane extracted sample. which mobile phase I should use?

09 September 2016 1,824 10 View