Jaesung Lee

6 Questions 6 Answers 0 Followers

Questions related from Jaesung Lee

Contradictory qRT-PCR results using different primers on identical cDNA template after ASO treatment targeting HER2 mRNA?

Hello colleagues researchers! I'm encountering puzzling results in my ASO (Antisense Oligonucleotide) experiment targeting HER2 mRNA and would appreciate your insights. Experimental Design: ASOs...

last month 4,371 4 View

Western Blot: Non-specific bands?

Hello, I have a questions about WB bands since I have almost no experience about this technique. I have done a WB for detecting a downregulation of HER2 protein on SK-BR-3(HER2 positive breast...

21 July 2025 6,644 4 View

QPCR Fluorescence Intensity Differences with Similar Ct Values – Issue for ΔΔCt Analysis?

As a preliminary experiment for qRT-PCR, I performed qPCR using synthetically ordered amplicons for GAPDH and HER2 as templates. Aside from differences in template and primer sequences, all...

12 March 2025 8,466 0 View

Can I use DTT instead of β-mercaptoethanol in RNA extraction lysis buffer?

Hello! I am using the AccuPrep® Universal RNA Extraction Kit(https://us.bioneer.com/pagecat1/accuprep/universal-rna-extraction-kit?tab=overview), which requires adding 10 µL of 99%...

19 February 2025 2,542 2 View

Protein-DNA conjugate recovery method?

Hello! I am the first one to try click chemistry on a lab so I need your help. I am planning to conjugate short peptide sequence(~20aa) with DNA(20~150nt) by click chemistry. There must be a...

24 December 2024 5,206 2 View

How can I measure the cytotoxicity induced by Cas9 Gene KO?

Hello! I am planning to KO a gene that seems critical to the cell's viability by CRISPR/Cas9. The question is, how can I detect the dead cells that are truly dead by KO by Cas9 when the gene is...

18 November 2024 1,112 6 View