Does anyone have any suggestions for making high molecular weight (~200kD) phospho-proteins show up better on Western Blot?

I've exhausted a lot of options to try and make phospho-ErbB4 (Cell Signaling) show up on my western blots. I've mainly tried using 3-8% Tris-Acetate gels, running in 1x Tricine (Biorad) for...

06 March 2018 4,540 12 View