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Questions related from Jacob Perkins
I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...
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I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...
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I am planning to sequence some genomic DNA with NGS and as a control/estimation of error in my workflow, I plan to spike in a synthetic oligo of ~100 bp (plus adaptors). At the center of it will...
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I am new to GC-MS and mass spec in general. Currently, I am attempting to quantify the uracil base content in cells by derivatizing it with 3,5-Bis(trifluoromethyl)benzyl bromide, where two of the...
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