Jackie Tan

3 Questions 4 Answers 0 Followers

Questions related from Jackie Tan

How do I determine whether the protein I heterologously expressed is lipidated?

Hello everyone, I am attempting to heterologously express a bacterial protein in E. coli. The protein contains a signal peptide which is cleaved and the N terminal cysteine is...

01 January 2016 4,492 4 View

Is there a chart where we can compare the migration speeds of a supercoiled uncut plasmid vs a linear cut plasmid of the same size?

I tried to insert a 6.6kbp gene into a 3.8kbp using CloneEZ, and transformed it into E. coli. After that a colony was selected and grown in 10mL of media for miniprep. I did a gel run...

08 August 2015 3,035 5 View

What are the possible strategies in single particle reconstruction using noisy data?

I´m trying to perform single particle reconstruction (SPR) of a protein of mine (around 190 kDa) using negative stain data. Normally, particles under negative stain look relatively well but this...

07 July 2015 2,234 2 View