Jack Staunton

4 Questions 31 Answers 0 Followers

Questions related from Jack Staunton

How to stain glycocalyx with fluorescence?

I am looking for a way to visualize the glycocalyx of live cells (preferably fluorescence by blue or red excitation). After googling, it appears that the standard method is to use periodic acid...

05 May 2013 3,193 5 View

What is a typical maximum field of view in TIRFM?

Using a 100X 1.45NA oil objective and an EM-CCD camera. In practice? In principle?

01 January 2013 3,186 1 View

Can anyone help me analyze a dataset from gene omnibus in matlab?

I have no experience with microarray data analysis, but I know my way around MATLAB pretty well. I am working on a project and I want to explore the microarray data published on the Gene...

10 October 2012 5,520 1 View

Can one select a stably transfected clone by FACS alone?

I want to transfect MCF-10A breast cells with the construct p^CMV LifeAct-TagGFP2: http://www.ibidi.com/products/reagents/R_6010X_pLifeAct.html I don't have any experience with transfection. I...

07 July 2012 1,328 9 View