Can anyone help with making a GFP plasmid to be integrated into a Crispr/cas9 mediated knock out site?

My goal is to be able to integrate a plasmid containing the GFP construct where there will be the desired cleavage from the Cas9. To do so, i did as described in the paper of  Auer T.O. et al...

09 September 2014 3,047 4 View