Ivan Duran

3 Questions 1 Answers 0 Followers

Questions related from Ivan Duran

Why can't I isolate single cell colonies after doing crispr in human cell line?

I have been introducing missense mutations in several human osteoblastic lines by transfecting vectors by the double nick method, ssODN and GFP reporter of specific crispr activity. I can see GFP...

07 July 2017 8,935 3 View

Does anybody know a good immortal chondrocyte cell line in humans?

I am looking for a chondrocyte cell line that I can stably transfect and differentiate in hypertrophic

12 December 2015 9,706 3 View

How can I visualize cilia in the growth plate?

What is the best method to stain and visualize cilia in a tissue like bone/cartilage, which have so strong autofluorescence?

09 September 2014 1,346 1 View