Ivan Pokrovac

2 Questions 1 Answers 0 Followers

Questions related from Ivan Pokrovac

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

04 November 2020 6,524 3 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

26 October 2020 8,782 1 View