Ivan Pokrovac

4 Questions 1 Answers 0 Followers

Questions related from Ivan Pokrovac

What is the best medium for short term storage of mouse testicular cells?

I have mouse testicular cells, from which I isolate spermatogonia (non-stem) using MACS. The buffer I use in MACS is 1xPBS supplemented with 0.5% BSA (bovine serum albumin) and 2mM EDTA. After...

10 March 2021 2,283 1 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

04 November 2020 6,635 3 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

26 October 2020 8,913 1 View

Is there FACS/MACS method based on isolation of high purity mouse spermatid cells that does not employ intercalating DNA dyes?

I am looking to isolate spermatid cells, as they are haploid cells that do not posses highly condensed chromatin of mature sperm cells. It is paramount that cells are minimally disturbed, and all...

30 August 2020 4,428 0 View