Isla Nair

8 Questions 9 Answers 0 Followers

Questions related from Isla Nair

If a gene is down-regulated does that still mean it is being expressed however the product it encodes for is decreased?

Hi all , I am confirming gene expression using RT-PCR. If a gene is down-regulated does that mean it is not expressed in the strain?

15 October 2019 5,664 3 View

How to interpret qPCR data and what statistical analysis to use?

Hi all , I am currently working with a hypothetical protein and I wanted to check if the gene encoding the protein is expressed in clinical strains by using quantitative PCR on cDNA synthesized...

12 October 2019 356 4 View

Is a Gene “conserved” it present in clinical strains ?

Hi all, I am working on character an unknown mtb gene. I performer pcr using gene specific primers on 6 clinical strains , bands around the correct size were visible. My question is : can I state...

10 October 2019 1,506 6 View

How to interpret qPCR data and what statistical analysis to use?

Hi all , I am currently working with a hypothetical protein and I wanted to check if the gene encoding the protein is expressed in clinical strains by using quantitative PCR on cDNA synthesized...

10 October 2019 2,313 5 View

How to I analyze gene expression using qpcr? And how do I report the Results?

Hi all. I am trying to determine if a gene is expressed in clinical strains. I was told to perform qpcr using cDNA synthesized from the RNA of those strains. My question is how do I analyze the...

10 October 2019 1,732 2 View

If a gene is down-regulated does that still mean it is being expressed however the product it encodes for is decreased?

Hi all , I am confirming gene expression using RT-PCR. If a gene is down-regulated does that mean it is not expressed in the strain?

10 October 2019 6,684 3 View

Mtb Integral Protein expression and extraction from BL21 cells?

Hi guys. I am currently trying to express a hypothetical protein that belongs to Mtb in an e.coli BL21 host cloned into a PGEX vector. Upon bioinformatic analysis it shows that this is an integral...

27 July 2019 5,034 6 View

Mtb Integral Protein expression and extraction from BL21 cells?

Hi guys. I am currently trying to express a hypothetical protein that belongs to Mtb in an e.coli BL21 host cloned into a PGEX vector. Upon bioinformatic analysis it shows that this is an integral...

07 July 2019 7,169 7 View