I am having problem with cloning a 2.2 kb fragment into pCMV Tag 2B vector using BamHI and EcoRI restriction sites. Any suggestions?

I want to clone a 2.2kb fragment in pCMV Tag 2B vector using BamHI and EcoRI. I usually do a PCR(My PCR gives 3 faint non specific bands above my desired product size). So I run a gel and then...

09 August 2016 4,344 8 View