Why my LNCaP clone FGC (ATCC® CRL-1740™) does not differentiate and does not attach to the flask after the first thaw?

I recently bought a LNCaP clone FGC (ATCC® CRL-1740™) vial and thawed it following all the steps described in datasheet. I used the recomended culture medium (RPMI-1640) and FBS at 10% (all from...

11 November 2020 5,405 3 View