Isabela Ichihara de Barros

2 Questions 6 Answers 0 Followers

Questions related from Isabela Ichihara de Barros

IPSC neural differentiation with poly-ornithine/laminin coated plate?

I am going to induce neural/astrocyte differentiation of my iPSC cells, but I have some doubts about the protocol (based on the article from Chailangkarn et al., 2016). Do I have necessarily to...

27 November 2018 7,928 8 View

Any tips for RACE PCR?

I'm trying to perform RACE PCR with the GeneRacer kit (Invitrogen). Since the first PCR I'm getting bands much smaller than the expected size for the 5' reaction. Also, I'm having trouble with the...

23 November 2018 8,670 3 View