Isabel Tundidor

2 Questions 4 Answers 0 Followers

Questions related from Isabel Tundidor

How to improve efficiency of CRISPR-Cas9 mediated knock out?

Apart from the Cas9-guide vector, I'm using a donor vector containing left and right homologous arms and a puromycin resistance casette. After antibiotic selection, resistant cells grow as...

03 April 2017 6,110 3 View

Which are B27 supplement components and why is it used for mammosphere formation?

Hello! I'm currently working with different breast cancer lines, from which I am trying to form mammospheres to asses CSC activity. I've read in several protocols that the addition of B27...

07 March 2017 1,835 3 View