Why I cannot detect gene expression by RT-qPCR, using SYBR Green, but I can observe the bands of the same products in an agarose gel?

I use different primers to amplify the same gene. The amplification is succesed on control samples, but in my experimental samples there appears to be no signal. When I run an agarose gel with the...

24 November 2016 3,918 5 View