Isaac Krasnopolsky

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Questions related from Isaac Krasnopolsky

For people doing top-down proteomics, what has been a reliable volatile buffer system in your preparation of intact protein analytes?

Attempting to produce spectra of SPDP (527 Da adduct on solvent exposed lysines) modified transferrin. I am experiencing solubility issues even at the recommended concentration of 10uM with a...

31 July 2018 3,926 2 View

Does anyone have a reproducible protocol for protein conjugation using Cys(Npys) TAT modified peptides?

Specifically, I am trying to conjugate a Cys (Npys) TAT modified peptide to an 80kD globular protein so the reaction conditions must be non-denaturing (no organic solvents like DMF, etc.) If you...

02 March 2018 6,889 0 View