Irshad Baig

6 Questions 40 Answers 0 Followers

Questions related from Irshad Baig

Does anyone know any software to predict 3D structure of ssDNA aptamer (30mer oligonucleotide)?

I am trying to study DNA (ligand)-Protein (receptor) Interaction by molecular docking, hence i need .pdb file format for DNA as ligand?. I would highly appreciate if you could please lend your...

11 November 2013 4,079 26 View

Could anyone suggest some reliable docking programme/online server to perform ssDNA aptamer-Protein docking?

I found a couple of higher affinity, Inhibitory (nM) ss DNA aptamers towards my target protein through DNA SELEX and now very keen to study the possible DNA aptamer-Protein interactions (binding...

10 October 2013 4,943 14 View

Could somebody please recommend to me some good ways to determine 'Mode of Enzyme Inhibition'?

I am working on some DNA aptamers and interested to evaluate the inhibition potency and mode of inhibition of this aptamers against particular target enzyme. Briefly, I would like to know a good...

06 June 2013 7,212 12 View

Could anyone please suggest me, how to obtain recombinant protein of high purity [Single Band] ?

I purify my protein of size 65 kD by affinity Chromatography using Ni-Charged chelating sepharose, followed by Gel filteration Chromatography using Superdex 200 HB 10/30 column (Amersham,...

01 January 2012 9,662 7 View

Could anyone describe the standard method to calculate enzyme specific activity?

Would it be correct, if at some fixed concentration of substrate increasing enzyme concentration till the enzyme attain saturation, and then by dividing the corresponding enzyme activity of a...

12 December 2011 4,027 4 View

Could any one please suggest me Molecular Docking Tools/Softwares ?

I want to find the binding modes/site of chemical inhibitors with the protein of interest..?

12 December 2011 8,746 15 View