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Questions related from Irka Nur Adriana Binte Irwan
My supervisor has instructed me to conduct colony PCR on B. subtilis 6633, however, I am skeptical about it because colony PCR is commonly used to determine if a vector is present, I don't think...
25 May 2022 9,091 3 View
In my case, I have extracted the pGLO plasmid from E. coli HB 101. I carried out restriction digestion and ligation using pGLO as my vector and my target gene as my insert to form a recombinant...
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I am conducting my Final Year Project which involves PCR, however, due to time constraints caused by clashes in my schedule, I can only run PCR the next day. In order to optimise time, can I mix...
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Hi, Im trying to obtain the alpha-amylase gene from E. coli 25922 & E. coli 8379 and ligate it with pGLO plasmid which would then be transformed into E. coli HB101 cells. Could anyone...
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