Which are the optimal conditions for phospho-MLKL detection in Western Blot?

I have tried to detect phospho-MLKL in a Western Blot using the positive control cells L929 treated with 100 ng/ml TNF alpha, 100 nM Smac mimetic and 30 uM Z-VAD for 6h and then...

04 April 2018 5,798 1 View