Hello i am working on micro RNA .I am facing a problem that if i keep my prepared reaction mixture overnight i get results .but no results in fresh?

I mix all the reaction suddenly power suddenly breaks down after the reaction run 20 min at annealing temperature 58 i kept it at -20. I run the same sample along with new at 60.8 annealing...

05 May 2018 1,371 0 View