How do I screen for IgM autoreactivity on Hep-2 cells?

I am trying to screen human B cell culture supernatants for autoreactivity on Hep-2 slides (IFA). The supernatants contain both IgG (~10μg/mL) and IgM (~60μg/mL) antibodies. There are several...

01 January 2016 3,078 1 View