Inte Vogt

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Questions related from Inte Vogt

What is the difference between a minigene and midigene? When do you call it a midigene?

Also, does anyone have an idea how big your insert can be in a 6 kb vector when you use the gibson assembly as the cloning method to create a midigen and where you only design a forward primer at...

18 January 2024 9,467 1 View

Is it possible to use the gibson cloning method to create a midigene, this for use for studying splicing variants?

Someone who can give advice on this? I wanna use a midigene for a exon-trap assay. I read in several articles (such as: Sangermano et al., 2017 https://doi.org/10.1101/gr.226621.117) that the...

18 November 2023 4,976 5 View

Can you use a 0.5 macfarland of the bacterium for a motility test in LB soft agar?

Purpose of the experiment: To investigate the mobility of the 3 bacterial strains and thus see if they are able to move towards the roots. • Positive control bacteria: E. coli • Negative control...

13 January 2022 2,255 0 View