Hi everyone, I would like to ask, If we lose some volume when harvest the supernatant for ELISA, should we top up to make all the same before dilute?

I do ELISA many times, and my result is not the same. I realize that when I harvested the supernatant, the volume each well was different. For example I stimulate cells with 200 ul media, when I...

09 January 2024 5,690 4 View