Insha Rasool

3 Questions 3 Answers 0 Followers

Questions related from Insha Rasool

Can I use HEK293T cell lysate for In vitro ubiquitination assay?

The homologue of the protein of my interest (POI) is short lived in yeast and the E3 ligase that targets it for degradation is also known. In case of mammalian system testing the half life of POI...

25 May 2018 3,494 1 View

Non-specificity in GST pull down from mammalian whole cell lysate

I have been doing pulldown with bacterial purified GST tagged protein as bait and HEK293T expressed Ha tagged TRIM family proteins as prey. I prepare whole cell lysate in a buffer containing mild...

12 July 2017 3,088 5 View

Reaction condition and buffers for detection of N-terminal deacetylase activity in mammalian cell extract

The buffer composition and reaction conditions compatible for HDACs and surtuin based deacetylation is optimised and well known. I want to check the in vitro N-terminal deacetylase activity so,...

06 October 2016 9,145 1 View