Insha Rasool

2 Questions 3 Answers 0 Followers

Questions related from Insha Rasool

Non-specificity in GST pull down from mammalian whole cell lysate

I have been doing pulldown with bacterial purified GST tagged protein as bait and HEK293T expressed Ha tagged TRIM family proteins as prey. I prepare whole cell lysate in a buffer containing mild...

12 July 2017 3,068 5 View

Reaction condition and buffers for detection of N-terminal deacetylase activity in mammalian cell extract

The buffer composition and reaction conditions compatible for HDACs and surtuin based deacetylation is optimised and well known. I want to check the in vitro N-terminal deacetylase activity so,...

06 October 2016 9,120 1 View