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The homologue of the protein of my interest (POI) is short lived in yeast and the E3 ligase that targets it for degradation is also known. In case of mammalian system testing the half life of POI...
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I have been doing pulldown with bacterial purified GST tagged protein as bait and HEK293T expressed Ha tagged TRIM family proteins as prey. I prepare whole cell lysate in a buffer containing mild...
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