Iñigo Lobara

5 Questions 6 Answers 0 Followers

Questions related from Iñigo Lobara

UV irradiated DNA stability?

Hi, I am working with Hela cells and my treatment condition is always UV light. One of the assays that I work all the time is ELISA on UV photoproducts, more specifically cyclobutane pyrimidine...

14 November 2018 1,390 1 View

0.5 mM EDTA; pH 9.0 & 10 mM Tris-Cl absorption at 492?

Hi, I was wondering how could I validate whether a solution with 0.5 mM EDTA; pH 9.0 & 10 mM Tris-Cl may have any absorbance values at 492 nm ( i rather mean if I can check it...

23 April 2018 1,639 2 View

0.5 mM EDTA; pH 9.0 & 10 mM Tris-Cl absorption at 492?

Hi, I was wondering how could I validate whether a solution with 0.5 mM EDTA; pH 9.0 & 10 mM Tris-Cl may have any absorbance values at 492 nm ( i rather mean if I can check it...

04 April 2018 9,886 2 View

ChIP crosslink removal?

Hi, I am performing a ChIP protocol. There i start with my cells in dishes, which I fix in FA%, the I quench the FA, lyse them and then I pellet the chromatin. Then I perform the...

26 April 2017 3,749 4 View

Fibroblast detachment after UV exposure?

Hi, I am performing UV irradiation studies on human fibroblasts. I want to perform IF after UV exposure and a short recovery time, which would follow pre - extraction at 4 degrees and PFA 4%...

21 March 2017 3,715 1 View